Application

Anti-JMJD2C Antibody, clone 6A3.1 is an antibody against JMJD2C for use in western blotting & ICC.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryChromatin Biology

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected JMJD2C in HeLa cells.

Alexa Fluor^® is a registered trademark of Life Technologies™.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

JMJD2C, also known as Lysine-specific demethylase 4C, and encoded by the gene KDM4C/ GASC1/ JHDM3C/ JMJD2C/ KIAA0780, is a histone demethylase that specifically demethylates ′Lys-9′ and ′Lys-36′ residues of histone H3, thereby playing a central role in histone code. JMJD2C does not demethylate histone H3 ′Lys-4′, H3 ′Lys-27′, nor H4 ′Lys-20′. JMJD2C can also demethylate trimethylated H3 ′Lys-9′ and H3 ′Lys-36′ residues, but JMJD2C has no activity on mono- and dimethylated residues. JMJD2C is overexpressed in several esophageal squamouse cancer cells and serves as a cancer marker for such cancers. This gene is also amplified in squamous cell carcinoma 1 protein (GASC-1). By demethylating H3K9me, JMJD2C/KDM4C acts as a transcription promoter on many target genes including genes the affect cell growth in normal or cancer cells. JMJD2C also regulates sphere formation by mediating the cross talk between Wnt and Notch pathways in colonic cancer cells and thus is a target for anti-cancer therapy for this disease.

Immunogen

GST-tagged recombinant protein corresponding to human JMJD2C.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected JMJD2C in 10 µg of HeLa neclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed